chromatography: Paper chromatography is part of the “fluid chromatography”. Here, the stationary phase consists of a paper strip, which stands vertical in a glass container, while the mobile phase consists of a liquid. The movement of the mobile phase is caused by the capillary forces. Applications for paper chromatography are in the analysis of mixtures of substances.
Thin layer chromatography: Thin layer chromatography is part of the “fluid chromatography” and operates the same way as paper chromatography. The difference between the two procedures lies in the stationary phase. In thin layer chromatography, this phase is built up of pulverised material such as aluminium oxide, silica gel or cellulose, which is applied to thin glass plates. The advantages of thin layer chromatography are the fast operating time and the high detection
Column chromatography: Column chromatography is part of the “fluid chromatography”. In column chromatography, the stationary phase mostly consists of powdery silica gel or aluminium oxide, which is added to a glass tube and then filled up with a solvent (mobile phase). In this process, the sample is brought together with the mobile phase in the glass tube. In doing so, the ingredients of the assay are separated and leave the tube one after another. A common application for column chromatography lies in the in purification of
Gas chromatography: In gas chromatography, the two following procedures are used:
The most frequently used procedure is the gas-fluid-chromatography procedure. During this procedure, the substrate (e.g. silicone oil) is filled into a spiralled tube with a diameter of 0, 1 – 5 mm and a length of up to 5 meters. The gas mixture which is to be analysed streams through the spiral together with the carrying gas (azote, helium, argon). At the end of the spiral, a heat conductance-detector measures the temperature variations. Out of these temperature variations, the material components can be deduced.